NAD+ Dosage Chart & Schedule
A coenzyme central to cellular energy metabolism and DNA-repair signaling. Levels decline with age, making it a major focus of longevity and mitochondrial research. Included here as a commonly co-studied research cofactor.
A coenzyme (not a peptide) studied across a wide range - references span roughly 100-500 mg. Subcutaneous injection commonly stings and is given slowly; larger amounts are usually delivered by slow IV in studies.
Figures describe how NAD+ is reported in the research literature and research-community references — not a recommendation for human use. For laboratory research only.
Dosage protocols
Commonly referenced research tiers. Start conservative; every compound and study context differs.
- Frequency
- 2-3x weekly
- Weekly total
- 100-150 mg
- Cycle
- Assess tolerability first
Low subcutaneous amount often used to gauge stinging and flushing before increasing.
- Frequency
- 2-3x weekly
- Weekly total
- 200-300 mg
- Cycle
- Ongoing or short course
Mid-range subcutaneous reference; injected slowly to reduce local stinging.
- Frequency
- Per protocol
- Weekly total
- Varies
- Cycle
- Study protocol
Larger references at the top of the range are typically delivered by slow IV rather than subQ due to volume and tolerability.
A cofactor, not a peptide
- Included as a commonly co-studied research cofactor; it is a coenzyme central to redox reactions and a substrate for sirtuins and PARPs.
- Rapidly metabolized, which is why oral precursors (NMN, NR) are studied separately for bioavailability.
Route and tolerability
- Subcutaneous injection commonly stings and is given slowly; larger amounts are usually infused by slow IV in study protocols.
- Fast administration is associated with flushing and chest discomfort, so slow delivery is emphasized.
Ready to run this protocol?
Dosing is only as good as the vial. NAD+ is third-party tested with published COAs from our top-rated source — reconstitute with confidence and skip the guesswork on identity and purity.
Weekly schedule
A typical week on the standard protocol (2-3x weekly).
Dosing day Off
NAD+ reconstitution
Based on a standard 500 mg vial: with 3 mL bacteriostatic water = 166,667 mcg/mL (166.7 mg/mL); 1 unit ≈ 1.7 mg.
From vial to injection in 6 steps
Wipe both vial tops
Swab the peptide vial and the bacteriostatic-water vial with fresh alcohol and let them dry.
Draw the water
Pull the planned amount of bacteriostatic water (3 mL) into the syringe.
Add it slowly
Run the water down the inside wall of the vial — don't blast the powder. Swirl gently; never shake.
Let it dissolve
Wait until the solution is fully clear, then refrigerate the vial.
Draw your dose
Pull the calculated units on a U-100 insulin syringe (~30 units for 50 mg).
Store between uses
Keep the reconstituted vial refrigerated and use within its stable window.
Handling & storage
- A large 500 mg vial + 3 mL bacteriostatic water = about 166.7 mg/mL, so 50 mg draws to roughly 30 units on a U-100 syringe.
- Inject slowly: rapid subcutaneous NAD+ is associated with stinging and flushing.
- A 250 mg or larger dose exceeds 1 mL at this concentration and is typically given by slow IV in studies rather than subQ.
- Refrigerate reconstituted solution and keep the lyophilized powder cool and dark.
NAD+ dosing FAQ
Why does NAD+ come in such a large vial?
References run much higher than most peptides (roughly 100-500 mg), so vials are sized in the hundreds of milligrams; a 500 mg vial in 3 mL gives about 166.7 mg/mL.
Why inject it slowly?
Rapid administration is commonly associated with stinging, flushing, and chest tightness, so both subQ and IV references emphasize slow delivery.
SubQ or IV?
Smaller references (about 50-100 mg) are drawn for slow subcutaneous injection; larger amounts (roughly 250-500 mg) are usually delivered by slow IV in study protocols.
Sources
Categories: Longevity & Cellular · Status: Research use only. This page is educational and for laboratory research use only; it is not medical advice.