Thymosin Beta-4 Dosage Chart & Schedule
Thymosin beta-4 is a naturally occurring 43-amino-acid peptide and a major intracellular actin-sequestering molecule. It is the full-length parent from which the research fragment commonly sold as TB-500 is derived, and it is studied for tissue repair, angiogenesis, and cell migration. It remains investigational.
In the research literature, the full-length thymosin beta-4 peptide is typically referenced at low-milligram subcutaneous doses (around 2-2.5 mg) given one to two times weekly across multi-week cycles, then tapered. Human injectable dosing is not established.
Figures describe how Thymosin Beta-4 is reported in the research literature and research-community references — not a recommendation for human use. For laboratory research only.
Dosage protocols
Commonly referenced research tiers. Start conservative; every compound and study context differs.
- Frequency
- Once weekly
- Weekly total
- 2 mg/week
- Cycle
- 4-6 week cycle
Lower end of the referenced range; sometimes used before a loading phase.
- Frequency
- Twice weekly
- Weekly total
- 4-5 mg/week
- Cycle
- 4-6 week loading phase
The most commonly referenced loading pattern for the full-length peptide.
- Frequency
- Once weekly or every other week
- Weekly total
- ~1-2.5 mg/week
- Cycle
- Ongoing after loading
Reduced frequency after an initial multi-week loading period.
Full-length peptide vs the TB-500 fragment
- Thymosin beta-4 is the complete 43-amino-acid parent molecule; TB-500 is a shorter actin-binding fragment derived from it.
- Pharmacokinetics of the full-length 43-mer differ from the fragment, so dosing references are not interchangeable.
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Weekly schedule
A typical week on the standard (loading) protocol (Twice weekly).
Dosing day Off
Thymosin Beta-4 reconstitution
10 mg vial + 2 mL bacteriostatic water = 5,000 mcg/mL (5 mg/mL); 1 unit is approximately 0.05 mg (50 mcg).
From vial to injection in 6 steps
Wipe both vial tops
Swab the peptide vial and the bacteriostatic-water vial with fresh alcohol and let them dry.
Draw the water
Pull the planned amount of bacteriostatic water (2 mL) into the syringe.
Add it slowly
Run the water down the inside wall of the vial — don't blast the powder. Swirl gently; never shake.
Let it dissolve
Wait until the solution is fully clear, then refrigerate the vial.
Draw your dose
Pull the calculated units on a U-100 insulin syringe (~50 units for 2.5 mg).
Store between uses
Keep the reconstituted vial refrigerated and use within its stable window.
Handling & storage
- Reconstitute with bacteriostatic water down the vial wall; do not shake, swirl gently to dissolve.
- A 10 mg vial with 2 mL yields 5 mg/mL, so 1 unit on a U-100 syringe is about 50 mcg.
- Store the sealed lyophilized vial refrigerated or frozen; keep the reconstituted vial refrigerated.
- Investigational research compound; not an approved drug.
Thymosin Beta-4 dosing FAQ
How much thymosin beta-4 per injection is referenced?
Research-community references for the full-length peptide commonly cite about 2-2.5 mg per subcutaneous injection, mirroring TB-500-style amounts.
How often is it dosed?
Most references use a loading phase of twice weekly for several weeks, then taper to once weekly or every other week. Reported half-life is on the order of hours, but the actin-related effects are studied over a longer window, which is why weekly dosing is common.
How do I reconstitute a 10 mg vial?
Adding 2 mL of bacteriostatic water gives 5 mg/mL. On a U-100 syringe that makes 1 unit about 50 mcg, so a 2.5 mg dose is 0.5 mL (50 units).
Is thymosin beta-4 the same as TB-500?
No. TB-500 is a shorter fragment of this parent peptide. They are studied for similar repair-related effects, but the dosing references are tracked separately.
Sources
Categories: Healing & Recovery · Status: Approved (see notes). This page is educational and for laboratory research use only; it is not medical advice.