Semaglutide Dosage Chart & Schedule
A long-acting GLP-1 receptor agonist, FDA-approved for type-2 diabetes and chronic weight management under brand names Ozempic, Wegovy, and Rybelsus. One of the most-studied incretin peptides.
Reported clinical use is a slow once-weekly titration - commonly referenced starting near 0.25 mg weekly and stepping up over months (up to about 2.4 mg weekly for weight management) to manage gastrointestinal tolerability.
Figures describe how Semaglutide is reported in the research literature and research-community references — not a recommendation for human use. For laboratory research only.
Dosage protocols
Commonly referenced research tiers. Start conservative; every compound and study context differs.
- Frequency
- Once weekly
- Weekly total
- 0.25 mg
- Cycle
- ~4 weeks, then titrate
Commonly referenced initiation dose, described as a tolerability lead-in rather than a therapeutic target.
- Frequency
- Once weekly
- Weekly total
- 1 mg
- Cycle
- Hold ~4 weeks per step
Mid-ladder step reached by roughly doubling every ~4 weeks (0.25 -> 0.5 -> 1 mg) as tolerated.
- Frequency
- Once weekly
- Weekly total
- 2.4 mg
- Cycle
- Ongoing
Common target maintenance dose cited for weight management in the approved regimen.
Titration is the point
- The stepwise increase is described in the literature as a way to limit nausea and other GI effects, not a dose-finding delay.
- An approximately 7-day half-life supports once-weekly administration.
Approved brands
- Marketed as Ozempic and Rybelsus (type-2 diabetes) and Wegovy (chronic weight management).
- Weight-management labeling references titration up to 2.4 mg weekly.
Ready to run this protocol?
Dosing is only as good as the vial. Research-grade peptides are third-party tested with published COAs from our top-rated source — reconstitute with confidence and skip the guesswork on identity and purity.
Weekly schedule
A typical week on the maintenance protocol (Once weekly).
Dosing day Off
Semaglutide reconstitution
Supplied as solution or lyophilized research material; a 5 mg vial + 2 mL bacteriostatic water = 2500 mcg/mL.
From vial to injection in 6 steps
Wipe both vial tops
Swab the peptide vial and the bacteriostatic-water vial with fresh alcohol and let them dry.
Draw the water
Pull the planned amount of bacteriostatic water (2 mL) into the syringe.
Add it slowly
Run the water down the inside wall of the vial — don't blast the powder. Swirl gently; never shake.
Let it dissolve
Wait until the solution is fully clear, then refrigerate the vial.
Draw your dose
Pull the calculated units on a U-100 insulin syringe (~10 units for 250 mcg).
Store between uses
Keep the reconstituted vial refrigerated and use within its stable window.
Handling & storage
- Lyophilized material: a 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL, so 0.25 mg draws to about 10 units on a U-100 syringe.
- At 2.5 mg/mL a 2.4 mg dose is nearly a full 1 mL syringe; a more concentrated mix (less water) keeps the injection volume small.
- Refrigerate after reconstitution and protect lyophilized powder from light and heat.
Semaglutide dosing FAQ
Why start at 0.25 mg?
Research and labeling describe 0.25 mg weekly as a tolerability lead-in to reduce gastrointestinal effects; it is generally not considered a maintenance dose.
How fast is the dose increased?
The commonly referenced schedule holds each step about 4 weeks before increasing (e.g., 0.25 -> 0.5 -> 1.0 -> 1.7 -> 2.4 mg weekly), adjusted to tolerability.
Is it dosed daily or weekly?
The injectable form is once weekly, consistent with its roughly 7-day half-life. An oral form (Rybelsus) is taken daily and dosed differently.
What draws up on a syringe?
From a 5 mg vial in 2 mL (2.5 mg/mL): 0.25 mg = about 10 units, 0.5 mg = about 20 units, 1 mg = about 40 units on a U-100 insulin syringe.
Sources
Categories: GLP-1 & Metabolic · Status: Approved (see notes). This page is educational and for laboratory research use only; it is not medical advice.