For laboratory research use only — not for human or veterinary consumption.
The Peptide Index
Dosing guide

HCG (Human Chorionic Gonadotropin) Dosage Chart & Schedule

HCG is a placental glycoprotein hormone that mimics luteinizing hormone and is FDA-approved for fertility indications and hypogonadotropic hypogonadism. In male research and TRT-adjacent contexts it is used to maintain testicular testosterone production and testicular size. It is supplied as a lyophilized powder dosed in international units (IU) rather than milligrams.

Route
Subcutaneous
Frequency
2-3x per week
Half-life
Biphasic
Storage
Reconstituted: refrigerate, use within weeks. Lyophilized: keep cool.
The short answer

HCG is dosed in international units (IU), not milligrams. For male testosterone support, research and clinical reports commonly cite roughly 250-500 IU subcutaneously two to three times weekly; ovulation induction instead uses a single dose near 5,000-10,000 IU. It is FDA-approved for fertility indications.

Figures describe how HCG (Human Chorionic Gonadotropin) is reported in the research literature and research-community references — not a recommendation for human use. For laboratory research only.

Dosage protocols

Commonly referenced research tiers. Start conservative; every compound and study context differs.

Testicular support (lower)
250 IU
At 2,500 IU/mL, 250 IU is about 0.1 mL (10 units on a U-100 syringe).
Frequency
2-3x per week
Weekly total
500-750 IU/week
Cycle
Ongoing (TRT-adjacent context)

Lower end used to help maintain testicular function and size.

Most commonTesticular support (standard)
500 IU
At 2,500 IU/mL, 500 IU is about 0.2 mL (20 units on a U-100 syringe).
Frequency
2-3x per week
Weekly total
1,000-1,500 IU/week
Cycle
Ongoing (TRT-adjacent context)

Commonly cited amount for maintaining Leydig-cell testosterone production.

Ovulation trigger (different use)
5,000-10,000 IU
A single large dose; a different clinical context from testicular support.
Frequency
Single dose
Weekly total
One-time
Cycle
Single administration

Used to trigger ovulation, not for ongoing testosterone support; included for contrast.

Dosed in IU, and it is an LH mimic

  • HCG binds the LH/CG receptor on testicular Leydig cells to drive testosterone synthesis, and in females triggers ovulation, so it is measured in international units, not milligrams.
  • It is FDA-approved for fertility indications and hypogonadotropic hypogonadism; half-life is biphasic (about 11 hours, then 24-36 hours), supporting a few-times-weekly schedule for testicular support.

Ready to run this protocol?

Dosing is only as good as the vial. Research-grade peptides are third-party tested with published COAs from our top-rated source — reconstitute with confidence and skip the guesswork on identity and purity.

Weekly schedule

A typical week on the testicular support (standard) protocol (2-3x per week).

Mon
Tue
Wed
Thu
Fri
Sat
Sun

Dosing day Off

From vial to injection in 6 steps

1

Wipe both vial tops

Swab the peptide vial and the bacteriostatic-water vial with fresh alcohol and let them dry.

2

Draw the water

Pull the planned amount of bacteriostatic water (per your mix) into the syringe.

3

Add it slowly

Run the water down the inside wall of the vial — don't blast the powder. Swirl gently; never shake.

4

Let it dissolve

Wait until the solution is fully clear, then refrigerate the vial.

5

Draw your dose

Pull the calculated units on a U-100 insulin syringe.

6

Store between uses

Keep the reconstituted vial refrigerated and use within its stable window.

Handling & storage

  • HCG is supplied lyophilized and measured in IU, not mg. A 5,000 IU vial with 2 mL of bacteriostatic water gives 2,500 IU/mL.
  • On a U-100 syringe 1 unit (0.01 mL) is about 25 IU, so a 250 IU dose is roughly 0.1 mL (10 units) and 500 IU is about 0.2 mL (20 units).
  • Reconstitute gently down the vial wall and keep the reconstituted vial refrigerated.
  • Track doses in IU rather than milligrams to avoid conversion errors.

HCG (Human Chorionic Gonadotropin) dosing FAQ

How is HCG dosed for testosterone support?

Reports commonly cite roughly 250-500 IU subcutaneously two to three times weekly to help maintain testicular testosterone production and size. It is dosed in IU, not milligrams.

How do I convert IU to syringe units?

Reconstituting a 5,000 IU vial with 2 mL gives 2,500 IU/mL. On a U-100 syringe that makes 1 unit about 25 IU, so 250 IU is about 10 units (0.1 mL) and 500 IU is about 20 units (0.2 mL).

Why is HCG measured in IU instead of mg?

As a glycoprotein hormone its activity is standardized biologically rather than by mass, so it is supplied and dosed in international units.

Is the ovulation dose the same as the testicular-support dose?

No. Ovulation induction uses a single large dose near 5,000-10,000 IU, which is a completely different context from the smaller 250-500 IU doses used a few times weekly for testicular support.

How long does HCG last in the body?

Its half-life is biphasic, roughly 11 hours initially, then a terminal phase of about 24-36 hours, which is why a few-times-weekly schedule is used for ongoing testicular support.

Sources

Full HCG (Human Chorionic Gonadotropin) reference →All dosing charts →

Categories: Sexual Health · Status: Approved (see notes). This page is educational and for laboratory research use only; it is not medical advice.