GHK-Cu Dosage Chart & Schedule
A naturally occurring copper-binding tripeptide whose plasma level declines with age. Extensively studied in skin biology for remodeling, and in topical cosmetic formulations.
Most human evidence is topical (0.05-2% formulations). Reconstituted research protocols commonly reference ~1-2 mg subQ daily or every other day in 4-8 week cycles. Injectable dosing is a research convention, not clinically established.
Figures describe how GHK-Cu is reported in the research literature and research-community references — not a recommendation for human use. For laboratory research only.
Dosage protocols
Commonly referenced research tiers. Start conservative; every compound and study context differs.
- Frequency
- 1-2x daily
- Weekly total
- Leave-on, per product
- Cycle
- Ongoing per formulation
Where most human cosmetic evidence sits: copper-peptide creams and serums for skin remodeling.
- Frequency
- Every other day
- Weekly total
- ~3-4 mg
- Cycle
- 4-8 week cycles
A conservative injectable entry point referenced while limiting cumulative copper load.
- Frequency
- Daily or EOD
- Weekly total
- ~7-14 mg
- Cycle
- 4-8 week cycles
The most commonly referenced reconstituted range; the solution appears faintly blue-green from the copper.
Strongest evidence is topical
- Most human GHK-Cu data comes from topical cosmetic formulations (0.05-2%) studied for skin remodeling and collagen synthesis.
- Injectable dosing is a research-community convention and is not clinically established.
Copper load is a consideration
- Because GHK carries a copper ion, cumulative copper exposure is a noted research consideration at higher or more frequent injectable doses.
- The reconstituted solution appears faintly blue-green, a visual cue of the bound copper.
Ready to run this protocol?
Dosing is only as good as the vial. GHK-Cu is third-party tested with published COAs from our top-rated source — reconstitute with confidence and skip the guesswork on identity and purity.
Weekly schedule
A typical week on the standard protocol (Daily or EOD).
Dosing day Off
GHK-Cu reconstitution
Based on a standard 50 mg vial: with 2 mL bacteriostatic water = 25,000 mcg/mL (25 mg/mL); 1 unit ≈ 250 mcg.
From vial to injection in 6 steps
Wipe both vial tops
Swab the peptide vial and the bacteriostatic-water vial with fresh alcohol and let them dry.
Draw the water
Pull the planned amount of bacteriostatic water (2 mL) into the syringe.
Add it slowly
Run the water down the inside wall of the vial — don't blast the powder. Swirl gently; never shake.
Let it dissolve
Wait until the solution is fully clear, then refrigerate the vial.
Draw your dose
Pull the calculated units on a U-100 insulin syringe (~4 units for 1 mg).
Store between uses
Keep the reconstituted vial refrigerated and use within its stable window.
Handling & storage
- Reconstitute with bacteriostatic water (2 mL into a 50 mg vial = 25,000 mcg/mL); solution appears faintly blue-green
- Refrigerate reconstituted; keep the lyophilized powder cool and dark
- A 1 mg dose draws to ~4 units on a U-100 insulin syringe (2 mg = ~8 units)
GHK-Cu dosing FAQ
How much GHK-Cu per injection?
Reconstituted research protocols commonly reference ~1-2 mg per subQ injection, daily or every other day in 4-8 week cycles. Human injectable dosing is not established.
How is GHK-Cu reconstituted?
With bacteriostatic water. For example, 2 mL into a 50 mg vial = 25,000 mcg/mL (25 mg/mL), so a 1 mg dose is about 4 units on a U-100 syringe. The solution turns faintly blue-green.
Is GHK-Cu used topically or injected?
Most human evidence is topical (0.05-2% creams and serums); injectable use is a research-community convention with less human data.
Why is the GHK-Cu solution blue-green?
The copper ion bound to the Gly-His-Lys tripeptide gives the reconstituted solution its characteristic faint blue-green color.
Sources
Categories: Cosmetic & Skin, Healing & Recovery · Status: Research use only. This page is educational and for laboratory research use only; it is not medical advice.